Data for discriminating dead/live bacteria in homogenous cell suspensions and the effect of insoluble substrates on turbidimetric measurements

Kwabena O. Duedu*, Christopher E. French

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    6 Citations (SciVal)

    Abstract

    Estimation of bacterial growth by rapid traditional methods such as spectrophometric measurements at 600 nm (OD600) is not applicable for cultures containing insoluble particles in the growth media. Colony counts are the only suitable alternative but these are laborious and not high-throughput. The data presented in this article is related to the research article entitled "Two-colour fluorescence fluorimetric analysis for direct quantification of bacteria and its application in monitoring bacterial growth in cellulose degradation systems" (Duedu and French, 2017) [1]. This data article presents original primary data describing the discrimination of dead/live bacteria in homogenous cell suspensions and how the presence of insoluble substrates affect the turbidity of the suspensions. (C) 2017 The Authors. Published by Elsevier Inc.
    Original languageEnglish
    Pages (from-to)169-174
    Number of pages6
    JournalData Brief
    Volume12
    DOIs
    Publication statusPublished (VoR) - 1 Jun 2017

    Keywords

    • Bacteria growth
    • Fluorimetry
    • Live/dead estimation
    • Propidium iodide
    • SYBR Green I nucleic acid gel stain

    Fingerprint

    Dive into the research topics of 'Data for discriminating dead/live bacteria in homogenous cell suspensions and the effect of insoluble substrates on turbidimetric measurements'. Together they form a unique fingerprint.

    Cite this