Gene doctoring: A method for recombineering in laboratory and pathogenic Escherichia coli strains

David J. Lee; Lewis Eh Bingle; Karin Heurlier; Mark J. Pallen; Charles W. Penn; Stephen Jw Busby; Jon L. Hobman

doi:10.1186/1471-2180-9-252

Gene doctoring: A method for recombineering in laboratory and pathogenic Escherichia coli strains

David J. Lee, Lewis Eh Bingle, Karin Heurlier, Mark J. Pallen, Charles W. Penn, Stephen Jw Busby, Jon L. Hobman

Research output: Contribution to journal › Article › peer-review

125 Citations (SciVal)

Original language	English
Article number	252
Journal	BMC Microbiology
Volume	9
DOIs	https://doi.org/10.1186/1471-2180-9-252
Publication status	Published (VoR) - 2009

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10.1186/1471-2180-9-252

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title = "Gene doctoring: A method for recombineering in laboratory and pathogenic Escherichia coli strains",

author = "Lee, {David J.} and Bingle, {Lewis Eh} and Karin Heurlier and Pallen, {Mark J.} and Penn, {Charles W.} and Busby, {Stephen Jw} and Hobman, {Jon L.}",

note = "Funding Information: The Authors would like to thank Prof. C Thomas (University of Birmingham) for the gift of the pEX100T plasmid, and Dr. T Overton (University of Birmingham) for the gift of the pSUB11 plasmid derivative carrying the 3 × FLAG sequence, used in the initial construction of the pDOC-K plasmid. This work was supported by a Wellcome Trust Programme Grant 076689 to SJWB, and BBSRC grant BB/E01044X/1 to CWP, JLH and MJP. The Birmingham Functional Genomics laboratory was supported by a Joint Infrastructure Fund grant JIF13209.",

year = "2009",

doi = "10.1186/1471-2180-9-252",

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journal = "BMC Microbiology",

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T1 - Gene doctoring

T2 - A method for recombineering in laboratory and pathogenic Escherichia coli strains

AU - Lee, David J.

AU - Bingle, Lewis Eh

AU - Heurlier, Karin

AU - Pallen, Mark J.

AU - Penn, Charles W.

AU - Busby, Stephen Jw

AU - Hobman, Jon L.

N1 - Funding Information: The Authors would like to thank Prof. C Thomas (University of Birmingham) for the gift of the pEX100T plasmid, and Dr. T Overton (University of Birmingham) for the gift of the pSUB11 plasmid derivative carrying the 3 × FLAG sequence, used in the initial construction of the pDOC-K plasmid. This work was supported by a Wellcome Trust Programme Grant 076689 to SJWB, and BBSRC grant BB/E01044X/1 to CWP, JLH and MJP. The Birmingham Functional Genomics laboratory was supported by a Joint Infrastructure Fund grant JIF13209.

PY - 2009

Y1 - 2009

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DO - 10.1186/1471-2180-9-252

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SN - 1471-2180

VL - 9

JO - BMC Microbiology

JF - BMC Microbiology

M1 - 252

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Gene doctoring: A method for recombineering in laboratory and pathogenic Escherichia coli strains

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