Gene doctoring: A method for recombineering in laboratory and pathogenic Escherichia coli strains

David J. Lee; Lewis Eh Bingle; Karin Heurlier; Mark J. Pallen; Charles W. Penn; Stephen Jw Busby; Jon L. Hobman

doi:10.1186/1471-2180-9-252

Gene doctoring: A method for recombineering in laboratory and pathogenic Escherichia coli strains

David J. Lee
, Lewis Eh Bingle
, Karin Heurlier
, Mark J. Pallen
, Charles W. Penn
, Stephen Jw Busby
, Jon L. Hobman

Research output: Contribution to journal › Article › peer-review

130 Citations (SciVal)

Original language	English
Article number	252
Journal	BMC Microbiology
Volume	9
DOIs	https://doi.org/10.1186/1471-2180-9-252
Publication status	Published (VoR) - 2009

Funding

The Authors would like to thank Prof. C Thomas (University of Birmingham) for the gift of the pEX100T plasmid, and Dr. T Overton (University of Birmingham) for the gift of the pSUB11 plasmid derivative carrying the 3 × FLAG sequence, used in the initial construction of the pDOC-K plasmid. This work was supported by a Wellcome Trust Programme Grant 076689 to SJWB, and BBSRC grant BB/E01044X/1 to CWP, JLH and MJP. The Birmingham Functional Genomics laboratory was supported by a Joint Infrastructure Fund grant JIF13209.

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10.1186/1471-2180-9-252

Cite this

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title = "Gene doctoring: A method for recombineering in laboratory and pathogenic Escherichia coli strains",

author = "Lee, \{David J.\} and Bingle, \{Lewis Eh\} and Karin Heurlier and Pallen, \{Mark J.\} and Penn, \{Charles W.\} and Busby, \{Stephen Jw\} and Hobman, \{Jon L.\}",

note = "Funding Information: The Authors would like to thank Prof. C Thomas (University of Birmingham) for the gift of the pEX100T plasmid, and Dr. T Overton (University of Birmingham) for the gift of the pSUB11 plasmid derivative carrying the 3 × FLAG sequence, used in the initial construction of the pDOC-K plasmid. This work was supported by a Wellcome Trust Programme Grant 076689 to SJWB, and BBSRC grant BB/E01044X/1 to CWP, JLH and MJP. The Birmingham Functional Genomics laboratory was supported by a Joint Infrastructure Fund grant JIF13209.",

year = "2009",

doi = "10.1186/1471-2180-9-252",

language = "English",

volume = "9",

journal = "BMC Microbiology",

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T1 - Gene doctoring

T2 - A method for recombineering in laboratory and pathogenic Escherichia coli strains

AU - Lee, David J.

AU - Bingle, Lewis Eh

AU - Heurlier, Karin

AU - Pallen, Mark J.

AU - Penn, Charles W.

AU - Busby, Stephen Jw

AU - Hobman, Jon L.

N1 - Funding Information: The Authors would like to thank Prof. C Thomas (University of Birmingham) for the gift of the pEX100T plasmid, and Dr. T Overton (University of Birmingham) for the gift of the pSUB11 plasmid derivative carrying the 3 × FLAG sequence, used in the initial construction of the pDOC-K plasmid. This work was supported by a Wellcome Trust Programme Grant 076689 to SJWB, and BBSRC grant BB/E01044X/1 to CWP, JLH and MJP. The Birmingham Functional Genomics laboratory was supported by a Joint Infrastructure Fund grant JIF13209.

PY - 2009

Y1 - 2009

UR - https://www.scopus.com/pages/publications/73449097174

UR - https://www.scopus.com/pages/publications/73449097174#tab=citedBy

U2 - 10.1186/1471-2180-9-252

DO - 10.1186/1471-2180-9-252

M3 - Article

C2 - 20003185

AN - SCOPUS:73449097174

SN - 1471-2180

VL - 9

JO - BMC Microbiology

JF - BMC Microbiology

M1 - 252

ER -

Gene doctoring: A method for recombineering in laboratory and pathogenic Escherichia coli strains

Funding

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