TY - JOUR
T1 - Genetic landscape for majority and minority HIV-1 drug resistance mutations in antiretroviral therapy naive patients in Accra, Ghana
AU - Appiah, Pious
AU - Gbassana, Gaspah
AU - Adusei-Poku, Mildred
AU - Obeng, Billal Musah
AU - Duedu, Kwabena Obeng
AU - Sagoe, Kwamena William Coleman
PY - 2024/6/19
Y1 - 2024/6/19
N2 - BackgroundThe successful detection of drug-resistance mutations (DRMs) in HIV-1 infected patients has improved the management of HIV infection. Next-generation sequencing (NGS) to detect low-frequency mutations is predicted to be useful for efficiently testing minority drug resistance mutations, which could contribute to virological failure. This study employed Sanger sequencing and NGS to detect and compare minority and majority drug resistance mutations in HIV-1 strains in treatment-naive patients from Ghana.
AB - BackgroundThe successful detection of drug-resistance mutations (DRMs) in HIV-1 infected patients has improved the management of HIV infection. Next-generation sequencing (NGS) to detect low-frequency mutations is predicted to be useful for efficiently testing minority drug resistance mutations, which could contribute to virological failure. This study employed Sanger sequencing and NGS to detect and compare minority and majority drug resistance mutations in HIV-1 strains in treatment-naive patients from Ghana.
KW - HIV drug resistance
KW - Minority drug-resistance mutations
KW - Next-generation sequencing
KW - Quasispecies
KW - Virological failure
UR - https://www.open-access.bcu.ac.uk/15602/
U2 - 10.1016/j.heliyon.2024.e33180
DO - 10.1016/j.heliyon.2024.e33180
M3 - Article
SN - 2405-8440
VL - 10
JO - Heliyon
JF - Heliyon
IS - 12
M1 - e33180
ER -