Genetic landscape for majority and minority HIV-1 drug resistance mutations in antiretroviral therapy naive patients in Accra, Ghana

Pious Appiah; Gaspah Gbassana; Mildred Adusei-Poku; Billal Musah Obeng; Kwabena Obeng Duedu; Kwamena William Coleman Sagoe

doi:10.1016/j.heliyon.2024.e33180

Genetic landscape for majority and minority HIV-1 drug resistance mutations in antiretroviral therapy naive patients in Accra, Ghana

Pious Appiah
, Gaspah Gbassana
, Mildred Adusei-Poku
, Billal Musah Obeng
, Kwabena Obeng Duedu
, Kwamena William Coleman Sagoe^*

^*Corresponding author for this work

University of Ghana
University of Liberia
University of New South Wales
University of Health and Allied Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

BackgroundThe successful detection of drug-resistance mutations (DRMs) in HIV-1 infected patients has improved the management of HIV infection. Next-generation sequencing (NGS) to detect low-frequency mutations is predicted to be useful for efficiently testing minority drug resistance mutations, which could contribute to virological failure. This study employed Sanger sequencing and NGS to detect and compare minority and majority drug resistance mutations in HIV-1 strains in treatment-naive patients from Ghana.

Original language	English
Article number	e33180
Journal	Heliyon
Volume	10
Issue number	12
DOIs	https://doi.org/10.1016/j.heliyon.2024.e33180
Publication status	Published (VoR) - 19 Jun 2024

Keywords

HIV drug resistance
Minority drug-resistance mutations
Next-generation sequencing
Quasispecies
Virological failure

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10.1016/j.heliyon.2024.e33180

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title = "Genetic landscape for majority and minority HIV-1 drug resistance mutations in antiretroviral therapy naive patients in Accra, Ghana",

abstract = "BackgroundThe successful detection of drug-resistance mutations (DRMs) in HIV-1 infected patients has improved the management of HIV infection. Next-generation sequencing (NGS) to detect low-frequency mutations is predicted to be useful for efficiently testing minority drug resistance mutations, which could contribute to virological failure. This study employed Sanger sequencing and NGS to detect and compare minority and majority drug resistance mutations in HIV-1 strains in treatment-naive patients from Ghana.",

keywords = "HIV drug resistance, Minority drug-resistance mutations, Next-generation sequencing, Quasispecies, Virological failure",

author = "Pious Appiah and Gaspah Gbassana and Mildred Adusei-Poku and Obeng, \{Billal Musah\} and Duedu, \{Kwabena Obeng\} and Sagoe, \{Kwamena William Coleman\}",

year = "2024",

month = jun,

day = "19",

doi = "10.1016/j.heliyon.2024.e33180",

language = "English",

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TY - JOUR

T1 - Genetic landscape for majority and minority HIV-1 drug resistance mutations in antiretroviral therapy naive patients in Accra, Ghana

AU - Appiah, Pious

AU - Gbassana, Gaspah

AU - Adusei-Poku, Mildred

AU - Obeng, Billal Musah

AU - Duedu, Kwabena Obeng

AU - Sagoe, Kwamena William Coleman

PY - 2024/6/19

Y1 - 2024/6/19

N2 - BackgroundThe successful detection of drug-resistance mutations (DRMs) in HIV-1 infected patients has improved the management of HIV infection. Next-generation sequencing (NGS) to detect low-frequency mutations is predicted to be useful for efficiently testing minority drug resistance mutations, which could contribute to virological failure. This study employed Sanger sequencing and NGS to detect and compare minority and majority drug resistance mutations in HIV-1 strains in treatment-naive patients from Ghana.

AB - BackgroundThe successful detection of drug-resistance mutations (DRMs) in HIV-1 infected patients has improved the management of HIV infection. Next-generation sequencing (NGS) to detect low-frequency mutations is predicted to be useful for efficiently testing minority drug resistance mutations, which could contribute to virological failure. This study employed Sanger sequencing and NGS to detect and compare minority and majority drug resistance mutations in HIV-1 strains in treatment-naive patients from Ghana.

KW - HIV drug resistance

KW - Minority drug-resistance mutations

KW - Next-generation sequencing

KW - Quasispecies

KW - Virological failure

UR - https://www.open-access.bcu.ac.uk/15602/

U2 - 10.1016/j.heliyon.2024.e33180

DO - 10.1016/j.heliyon.2024.e33180

M3 - Article

SN - 2405-8440

VL - 10

JO - Heliyon

JF - Heliyon

IS - 12

M1 - e33180

ER -

Genetic landscape for majority and minority HIV-1 drug resistance mutations in antiretroviral therapy naive patients in Accra, Ghana

Abstract

Keywords

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