Hakai is required for stabilization of core components of the m6A mRNA methylation machinery

Praveen Bawankar; Tina Lence; Chiara Paolantoni; Irmgard U. Haussmann; Migle Kazlauskiene; Dominik Jacob; Jan B. Heidelberger; Florian M. Richter; Mohanakarthik P. Nallasivan; Violeta Morin; Nastasja Kreim; Petra Beli; Mark Helm; Martin Jinek; Matthias Soller; Jean Yves Roignant

doi:10.1038/s41467-021-23892-5

Hakai is required for stabilization of core components of the m⁶A mRNA methylation machinery

Praveen Bawankar, Tina Lence, Chiara Paolantoni, Irmgard U. Haussmann, Migle Kazlauskiene, Dominik Jacob, Jan B. Heidelberger, Florian M. Richter, Mohanakarthik P. Nallasivan, Violeta Morin, Nastasja Kreim, Petra Beli, Mark Helm, Martin Jinek, Matthias Soller, Jean Yves Roignant^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

124 Citations (SciVal)

Original language	English
Article number	3778
Journal	Nature Communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-23892-5
Publication status	Published (VoR) - Dec 2021

Funding

We thank the Bloomington, Kyoto and Vienna stock Center for fly lines and the Drosophila Genomics Resource Center at Indiana University for plasmids and cell lines. We are indebted to S. Hayashi for his effort to recover the Hakai1 allele as well as BestGene and the University of Cambridge Department of Genetics Fly Facility for injections. We thank members of the Roignant and Soller lab for helpful discussion. We thank Christian Renz from the Ulrich group at IMB for sharing reagents and advices for the yeast-two-hybrid assay and Catia Igreja and Heike Budde from late Elisa Izaurralde lab for sharing plasmids used in this study. We thank the IMB Genomics core facility for their helpful support and the use of its NextSeq500 (INST 247/870-1 FUGG). Research in the laboratory of J.-Y.R. is supported by University of Lausanne, the Swiss National Science Foundation (310030_197906), the Deutsche Forschungsgemeinschaft RO 4681/9-1, RO 4681/12-1 and RO 4681/13-1. M.S. is funded by the BBSRC (BB/R002932/1) and the Leverhulme Trust. Research in the M.J. laboratory is supported by the Swiss National Competence Center for Research (NCCR) RNA & Disease. M.K. is supported by EMBO (ALTF 1087-2018) and Human Frontier Science Program (LT000248 2019-L) postdoctoral fellowships. M.J. is an International Research Scholar of the Howard Hughes Medical Institute and Vallee Scholar of the Bert L & N Kuggie Vallee Foundation. P.Beli is supported by the Emmy Noether Program (BE 5342/1-1 and BE 5342/1-2). C.P. in the lab of J.-Y.R. is supported by a Boehringer Ingelheim Fonds fellowship.

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Bawankar, P., Lence, T., Paolantoni, C., Haussmann, I. U., Kazlauskiene, M., Jacob, D., Heidelberger, J. B., Richter, F. M., Nallasivan, M. P., Morin, V., Kreim, N., Beli, P., Helm, M., Jinek, M., Soller, M., & Roignant, J. Y. (2021). Hakai is required for stabilization of core components of the m⁶A mRNA methylation machinery. Nature Communications, 12(1), Article 3778. https://doi.org/10.1038/s41467-021-23892-5

@article{94a50568ee58468fa95b1c994ecf4e83,

title = "Hakai is required for stabilization of core components of the m6A mRNA methylation machinery",

author = "Praveen Bawankar and Tina Lence and Chiara Paolantoni and Haussmann, \{Irmgard U.\} and Migle Kazlauskiene and Dominik Jacob and Heidelberger, \{Jan B.\} and Richter, \{Florian M.\} and Nallasivan, \{Mohanakarthik P.\} and Violeta Morin and Nastasja Kreim and Petra Beli and Mark Helm and Martin Jinek and Matthias Soller and Roignant, \{Jean Yves\}",

note = "Funding Information: We thank the Bloomington, Kyoto and Vienna stock Center for fly lines and the Drosophila Genomics Resource Center at Indiana University for plasmids and cell lines. We are indebted to S. Hayashi for his effort to recover the Hakai1 allele as well as BestGene and the University of Cambridge Department of Genetics Fly Facility for injections. We thank members of the Roignant and Soller lab for helpful discussion. We thank Christian Renz from the Ulrich group at IMB for sharing reagents and advices for the yeast-two-hybrid assay and Catia Igreja and Heike Budde from late Elisa Izaurralde lab for sharing plasmids used in this study. We thank the IMB Genomics core facility for their helpful support and the use of its NextSeq500 (INST 247/870-1 FUGG). Research in the laboratory of J.-Y.R. is supported by University of Lausanne, the Swiss National Science Foundation (310030\_197906), the Deutsche Forschungsgemeinschaft RO 4681/9-1, RO 4681/12-1 and RO 4681/13-1. M.S. is funded by the BBSRC (BB/R002932/1) and the Leverhulme Trust. Research in the M.J. laboratory is supported by the Swiss National Competence Center for Research (NCCR) RNA \& Disease. M.K. is supported by EMBO (ALTF 1087-2018) and Human Frontier Science Program (LT000248 2019-L) postdoctoral fellowships. M.J. is an International Research Scholar of the Howard Hughes Medical Institute and Vallee Scholar of the Bert L \& N Kuggie Vallee Foundation. P.Beli is supported by the Emmy Noether Program (BE 5342/1-1 and BE 5342/1-2). C.P. in the lab of J.-Y.R. is supported by a Boehringer Ingelheim Fonds fellowship. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1038/s41467-021-23892-5",

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Bawankar, P, Lence, T, Paolantoni, C, Haussmann, IU, Kazlauskiene, M, Jacob, D, Heidelberger, JB, Richter, FM, Nallasivan, MP, Morin, V, Kreim, N, Beli, P, Helm, M, Jinek, M, Soller, M & Roignant, JY 2021, 'Hakai is required for stabilization of core components of the m⁶A mRNA methylation machinery', Nature Communications, vol. 12, no. 1, 3778. https://doi.org/10.1038/s41467-021-23892-5

TY - JOUR

T1 - Hakai is required for stabilization of core components of the m6A mRNA methylation machinery

AU - Bawankar, Praveen

AU - Lence, Tina

AU - Paolantoni, Chiara

AU - Haussmann, Irmgard U.

AU - Kazlauskiene, Migle

AU - Jacob, Dominik

AU - Heidelberger, Jan B.

AU - Richter, Florian M.

AU - Nallasivan, Mohanakarthik P.

AU - Morin, Violeta

AU - Kreim, Nastasja

AU - Beli, Petra

AU - Helm, Mark

AU - Jinek, Martin

AU - Soller, Matthias

AU - Roignant, Jean Yves

N1 - Funding Information: We thank the Bloomington, Kyoto and Vienna stock Center for fly lines and the Drosophila Genomics Resource Center at Indiana University for plasmids and cell lines. We are indebted to S. Hayashi for his effort to recover the Hakai1 allele as well as BestGene and the University of Cambridge Department of Genetics Fly Facility for injections. We thank members of the Roignant and Soller lab for helpful discussion. We thank Christian Renz from the Ulrich group at IMB for sharing reagents and advices for the yeast-two-hybrid assay and Catia Igreja and Heike Budde from late Elisa Izaurralde lab for sharing plasmids used in this study. We thank the IMB Genomics core facility for their helpful support and the use of its NextSeq500 (INST 247/870-1 FUGG). Research in the laboratory of J.-Y.R. is supported by University of Lausanne, the Swiss National Science Foundation (310030_197906), the Deutsche Forschungsgemeinschaft RO 4681/9-1, RO 4681/12-1 and RO 4681/13-1. M.S. is funded by the BBSRC (BB/R002932/1) and the Leverhulme Trust. Research in the M.J. laboratory is supported by the Swiss National Competence Center for Research (NCCR) RNA & Disease. M.K. is supported by EMBO (ALTF 1087-2018) and Human Frontier Science Program (LT000248 2019-L) postdoctoral fellowships. M.J. is an International Research Scholar of the Howard Hughes Medical Institute and Vallee Scholar of the Bert L & N Kuggie Vallee Foundation. P.Beli is supported by the Emmy Noether Program (BE 5342/1-1 and BE 5342/1-2). C.P. in the lab of J.-Y.R. is supported by a Boehringer Ingelheim Fonds fellowship. Publisher Copyright: © 2021, The Author(s).

PY - 2021/12

Y1 - 2021/12

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U2 - 10.1038/s41467-021-23892-5

DO - 10.1038/s41467-021-23892-5

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C2 - 34145251

AN - SCOPUS:85108158058

VL - 12

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 3778

ER -

Hakai is required for stabilization of core components of the m⁶A mRNA methylation machinery

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