Abstract
Lignin-scribed graphene (LSG) conjugated with nitrogen-doped graphene quantum dots (N-GQDs) and lignin derived silver nanoparticles (Ag NPs) was developed through a hydrothermal process for the electrochemical sensing of Troponin I, a cardiac biomarker for Acute Myocardial Infarction (AMI). A nanocomposite with optimal conduction mechanism was developed by varying the N-GQDs doped amount intercalated on the surface of LSG. The nanocomposite was characterised by morphological, physical, and structural examinations. The Ag NPs and N-GQDs were found uniformly distributed on the LSG surface, with selective capture of the biotinylated aptamer probe on the bio-electrode indicative of the specific interaction with Troponin I, resulting in an increment in the charge transfer resistance following hybridisation analysis. The detection limit, as determined through impedance spectroscopy, was 1 fM or 30 fg/mL, with high levels of linearity, selectivity, repeatability, and stability of the sensor. This nanocomposite opens a new avenue for array-based medical diagnostics.
Original language | English |
---|---|
Article number | 109405 |
Journal | Microchemical Journal |
Volume | 195 |
DOIs | |
Publication status | Published (VoR) - 23 Sept 2023 |
Funding
The authors acknowledge the Ministry of Higher Education Malaysia for supporting the research with Fundamental Research Grant Scheme (FRGS) (FRGS/1/2022/TK09/UTP/02/3) and Universiti Teknologi PETRONAS (UTP) for the opportunity to carry out the research in Nanotechnology Research Laboratory and Dye Solar Cell Laboratory. The authors acknowledge the fund from Researchers Supporting Project Number (RSP2023R143), King Saud University, Riyadh, Saudi Arabia.
Funders | Funder number |
---|---|
King Saud University | |
Ministry of Higher Education, Malaysia | FRGS/1/2022/TK09/UTP/02/3 |
Universiti Teknologi Petronas | RSP2023R143 |
Keywords
- lignin-derived graphene
- nitrogen-doped graphene quantum dots
- silver nanoparticles
- troponin I
- biosensor