Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor RbM15/spenito to the m6 a machinery component Wtap/Fl(2)d

Philip Knuckles; Tina Lence; Irmgard U. Haussmann; Dominik Jacob; Nastasja Kreim; Sarah H. Carl; Irene Masiello; Tina Hares; Rodrigo Villaseñor; Daniel Hess; Miguel A. Andrade-Navarro; Marco Biggiogera; Mark Helm; Matthias Soller; Marc Bühler; Jean Yves Roignant

doi:10.1101/gad.309146.117

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor RbM15/spenito to the m⁶ a machinery component Wtap/Fl(2)d

Philip Knuckles, Tina Lence, Irmgard U. Haussmann, Dominik Jacob, Nastasja Kreim, Sarah H. Carl, Irene Masiello, Tina Hares, Rodrigo Villaseñor, Daniel Hess, Miguel A. Andrade-Navarro, Marco Biggiogera, Mark Helm, Matthias Soller, Marc Bühler, Jean Yves Roignant^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

501 Citations (SciVal)

Original language	English
Pages (from-to)	415-429
Number of pages	15
Journal	Genes and Development
Volume	32
Issue number	5-6
DOIs	https://doi.org/10.1101/gad.309146.117
Publication status	Published (VoR) - 1 Mar 2018

Funding

We thank the Bloomington Drosophila Stock Center and the Vienna Drosophila Resource Center for fly reagents, the Drosophila Genomics Resource Center at Indiana University for plasmids, members of the Bühler and Roignant laboratories for helpful discussion, Vytautas Iesmantavicius from the Protein Analysis Facility at Friedrich Miescher Institute for help with MS data analysis, and the Genomics and Bioinformatics Institute of Molecular Biology (IMB) Core Facilities for great support. Support by the IMB Proteomics Core Facility is gratefully acknowledged (the instrument is funded by Deutsche Forschungsgemeinschaft INST 247/766-1 FUGG). In particular, we thank Anja Freiwald from the IMB Proteomics Core Facility for sample preparation, and Dr. Mario Dejung from the Proteomics Core Facility for data processing and generating the scatter plot. Research in the laboratory of J.-Y.R. is supported by the Deutsche Forschungsge-meinschaft (DFG) RO 4681/5-1 and the Epitran COST action (CA16120). Work performed in M. Bühler’s laboratory was supported by the Novartis Research Foundation and the Swiss Science Foundation National Centre of Competence in Research RNA and Disease (grant no. 141735). Work performed in M.S.’s laboratory was supported by the Biotechnology and Biological Sciences Research Council. The project was also supported by the DFG SPP1784 grant to M.H.

Keywords

Flacc
M A
Methyltransferase complex
RNA modifications
Sex determination
Zc3h13

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10.1101/gad.309146.117

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Knuckles, P., Lence, T., Haussmann, I. U., Jacob, D., Kreim, N., Carl, S. H., Masiello, I., Hares, T., Villaseñor, R., Hess, D., Andrade-Navarro, M. A., Biggiogera, M., Helm, M., Soller, M., Bühler, M., & Roignant, J. Y. (2018). Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor RbM15/spenito to the m⁶ a machinery component Wtap/Fl(2)d. Genes and Development, 32(5-6), 415-429. https://doi.org/10.1101/gad.309146.117

@article{788ff2cb21af413fb187c611a32687c2,

title = "Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor RbM15/spenito to the m6 a machinery component Wtap/Fl(2)d",

keywords = "Flacc, M A, Methyltransferase complex, RNA modifications, Sex determination, Zc3h13",

author = "Philip Knuckles and Tina Lence and Haussmann, \{Irmgard U.\} and Dominik Jacob and Nastasja Kreim and Carl, \{Sarah H.\} and Irene Masiello and Tina Hares and Rodrigo Villase{\~n}or and Daniel Hess and Andrade-Navarro, \{Miguel A.\} and Marco Biggiogera and Mark Helm and Matthias Soller and Marc B{\"u}hler and Roignant, \{Jean Yves\}",

note = "Funding Information: We thank the Bloomington Drosophila Stock Center and the Vienna Drosophila Resource Center for fly reagents, the Drosophila Genomics Resource Center at Indiana University for plasmids, members of the B{\"u}hler and Roignant laboratories for helpful discussion, Vytautas Iesmantavicius from the Protein Analysis Facility at Friedrich Miescher Institute for help with MS data analysis, and the Genomics and Bioinformatics Institute of Molecular Biology (IMB) Core Facilities for great support. Support by the IMB Proteomics Core Facility is gratefully acknowledged (the instrument is funded by Deutsche Forschungsgemeinschaft Funding Information: INST 247/766-1 FUGG). In particular, we thank Anja Freiwald from the IMB Proteomics Core Facility for sample preparation, and Dr. Mario Dejung from the Proteomics Core Facility for data processing and generating the scatter plot. Research in the laboratory of J.-Y.R. is supported by the Deutsche Forschungsge-meinschaft (DFG) RO 4681/5-1 and the Epitran COST action (CA16120). Work performed in M. B{\"u}hler{\textquoteright}s laboratory was supported by the Novartis Research Foundation and the Swiss Science Foundation National Centre of Competence in Research RNA and Disease (grant no. 141735). Work performed in M.S.{\textquoteright}s laboratory was supported by the Biotechnology and Biological Sciences Research Council. The project was also supported by the DFG SPP1784 grant to M.H. Publisher Copyright: {\textcopyright} 2018 Knuckles et al.",

year = "2018",

month = mar,

day = "1",

doi = "10.1101/gad.309146.117",

language = "English",

volume = "32",

pages = "415--429",

journal = "Genes and Development",

issn = "0890-9369",

publisher = "Cold Spring Harbor Laboratory Press",

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Knuckles, P, Lence, T, Haussmann, IU, Jacob, D, Kreim, N, Carl, SH, Masiello, I, Hares, T, Villaseñor, R, Hess, D, Andrade-Navarro, MA, Biggiogera, M, Helm, M, Soller, M, Bühler, M & Roignant, JY 2018, 'Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor RbM15/spenito to the m⁶ a machinery component Wtap/Fl(2)d', Genes and Development, vol. 32, no. 5-6, pp. 415-429. https://doi.org/10.1101/gad.309146.117

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T1 - Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor RbM15/spenito to the m6 a machinery component Wtap/Fl(2)d

AU - Knuckles, Philip

AU - Lence, Tina

AU - Haussmann, Irmgard U.

AU - Jacob, Dominik

AU - Kreim, Nastasja

AU - Carl, Sarah H.

AU - Masiello, Irene

AU - Hares, Tina

AU - Villaseñor, Rodrigo

AU - Hess, Daniel

AU - Andrade-Navarro, Miguel A.

AU - Biggiogera, Marco

AU - Helm, Mark

AU - Soller, Matthias

AU - Bühler, Marc

AU - Roignant, Jean Yves

N1 - Funding Information: We thank the Bloomington Drosophila Stock Center and the Vienna Drosophila Resource Center for fly reagents, the Drosophila Genomics Resource Center at Indiana University for plasmids, members of the Bühler and Roignant laboratories for helpful discussion, Vytautas Iesmantavicius from the Protein Analysis Facility at Friedrich Miescher Institute for help with MS data analysis, and the Genomics and Bioinformatics Institute of Molecular Biology (IMB) Core Facilities for great support. Support by the IMB Proteomics Core Facility is gratefully acknowledged (the instrument is funded by Deutsche Forschungsgemeinschaft Funding Information: INST 247/766-1 FUGG). In particular, we thank Anja Freiwald from the IMB Proteomics Core Facility for sample preparation, and Dr. Mario Dejung from the Proteomics Core Facility for data processing and generating the scatter plot. Research in the laboratory of J.-Y.R. is supported by the Deutsche Forschungsge-meinschaft (DFG) RO 4681/5-1 and the Epitran COST action (CA16120). Work performed in M. Bühler’s laboratory was supported by the Novartis Research Foundation and the Swiss Science Foundation National Centre of Competence in Research RNA and Disease (grant no. 141735). Work performed in M.S.’s laboratory was supported by the Biotechnology and Biological Sciences Research Council. The project was also supported by the DFG SPP1784 grant to M.H. Publisher Copyright: © 2018 Knuckles et al.

PY - 2018/3/1

Y1 - 2018/3/1

KW - Flacc

KW - M A

KW - Methyltransferase complex

KW - RNA modifications

KW - Sex determination

KW - Zc3h13

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DO - 10.1101/gad.309146.117

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AN - SCOPUS:85044731834

SN - 0890-9369

VL - 32

SP - 415

EP - 429

JO - Genes and Development

JF - Genes and Development

IS - 5-6

ER -

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor RbM15/spenito to the m⁶ a machinery component Wtap/Fl(2)d

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Keywords

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